Journal: Antibiotics

Article Title: Evaluation of Biotechnological Active Peptides Secreted by Saccharomyces cerevisiae with Potential Skin Benefits

doi: 10.3390/antibiotics13090881

Figure Lengend Snippet: Cytotoxicity of the peptides (2–10 kDa) in melanoma cell line MNT1 and normal dermal fibroblasts, keratinocytes, and melanocytes. Cells were exposed to increasing concentrations of the peptide for 48 h and viability was evaluated with the MTS assay. Bars represent the average ± standard deviation.

Article Snippet: The medium for melanocytes and keratinocytes was Dermal cells basal media (PCS-200-030, ATCC) supplemented with melanocytes growth kit (PCS-200-041, ATCC) or keratinocytes growth kit (PCS-200-040, ATCC), respectively.

Techniques: MTS Assay, Standard Deviation

Journal: The American journal of pathology

Article Title: Slug expression during melanoma progression.

doi: 10.1016/j.ajpath.2012.02.014

Figure Lengend Snippet: Figure 3. Endogenous Slug expression is higher at both mRNA and protein levels in NHM than in primary (WM115) or metastatic (WC62) melanoma cell lines. A: RT-qPCR revealed significantly higher expression of Slug and E- cadherin mRNAs in NHM than in primary or metastatic melanoma cells, whereas Snail and N-cadherin expression were significantly lower. Error bars indicate SDs. *P 0.01 compared to both primary and metastatic melanoma cells. B: Western blots confirmed that differences in mRNA levels were reflected in differences in protein expression in NHM versus primary and metastatic melanoma cell lines. Both mRNA and protein were extracted from confluent cell cultures to eliminate density-dependent variations in expres- sion of Slug and its putative target genes. MITF isoforms include both melanocyte-specific (M) and ubiquitous (A) isoforms.

Article Snippet: Normal human melanocytes (NHM) of neonatal origin were obtained from the ATCC (Manassas, VA) and were maintained in Dermal Basal Medium supplemented with a melanocyte growth kit (ATCC).

Techniques: Expressing, Quantitative RT-PCR, Western Blot

Journal: The American journal of pathology

Article Title: Slug expression during melanoma progression.

doi: 10.1016/j.ajpath.2012.02.014

Figure Lengend Snippet: Figure 4. NHM and melanoma cells transduced with Slug-expressing adenovirus (Ad-Slug) express less E-cadherin and more N-cadherin mRNA and protein than cells transduced with a control adeno- virus (Ad-null). A: RT-qPCR revealed significantly lower E-cadherin expression in NHM transduced with Ad-Slug compared to Ad-Null-transduced cells. Neither Ad-Slug nor Ad-Null-transfected WC62 cells expressed detectable E-cadherin mRNA. N-cadherin levels were significantly increased in Ad-Slug-trans- duced NHM and melanoma cells compared to Ad- Null-transduced cells, although basal levels of ex- pression were low and increases were modest. Note the difference in scale for the two graphs. Error bars indicate SEMs. *P 0.03. B: Western blot results confirmed that Ad-Slug enhanced Slug protein ex- pression in NHM and melanoma cells and that the differences seen in mRNA levels for E-cadherin in NHM and N-cadherin in melanoma cells were re- flected in differences in protein expression. In addi- tion, Ad-Slug transduction also enhanced expression of melanocyte-specific MITF (M) in NHM but not in melanoma cells. Melanoma cells expressed higher levels of the MITF isoform constitutively expressed in many tissues (A) and expression of this isoform was not altered by Ad-Slug transduction. Ad-Slug transduction did not alter levels of Snail expression in either cell type.

Article Snippet: Normal human melanocytes (NHM) of neonatal origin were obtained from the ATCC (Manassas, VA) and were maintained in Dermal Basal Medium supplemented with a melanocyte growth kit (ATCC).

Techniques: Transduction, Expressing, Control, Virus, Quantitative RT-PCR, Transfection, Western Blot